| About the author: |
R.V. Fafula, О.K. Оnufrovych, U.P. Iefremova, M.Z. Vorobets, I.A. Nakonechnyi, O.V. Melnyk, Z.Ya. Fedorovych, Z.D. Vorobets |
| Heading |
CLINICAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
Hyperproduction of reactive oxygen species and disturbances of prooxidant/antioxidant balance can damage sperm cells and are considered as possible mechanisms of male infertility. The aim of present paper was to study the prooxidant/antioxidant balance in sperm cells of infertile men with different forms of pathospermia. In this study, we found the levels of intracellular TBARS in spermatozoa to be elevated and activity of glutathione antioxidant protection system (GPx, GR, GsT) and GSH content to be decreased in infertile individuals with different forms of pathospermia compared with the healthy man with normozoospermia. The index of antioxidant status was calculated by dividing the sum of the activities of antioxidant enzymes (GPx, GR, GsT) and GSH content to the sum of the TBARS and GSSG level. The extent of decrease of indexes of antioxidant status of spermatozoa of infertile men revealed a disorder-associated trend: oligozoospermia > oligoasthenozoospermia ≈ asthenozoospermia >> leucocytospermia. Unidirectional change in the index of antioxidant status in different forms of pathospermia allows to use it as biotest for intensity free radicals processes and resistance to free radicals in spermatozoa. |
| Tags |
antioxidant enzymes, lipid peroxidation, male infertility, pathospermia |
| Bibliography |
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| Publication of the article |
«World of Medicine and Biology» №4(66), 2018 year, 120-124 pages, index UDK 616.697-02:616.69-007.5 |
| DOI |
10.26724/2079-8334-2018-4-66-120-124 |
