Sviridyuk R.V., Shepitko V.I., Shepitko K.V., Klypachenko I.V.

CHARACTERISTICS OF THE SCIATIC NERVE TRUNK STRUCTURAL COMPONENTS WITH ADMINISTERING THE CRYOPRESERVED PLACENT IN RATS

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About the author:	Sviridyuk R.V., Shepitko V.I., Shepitko K.V., Klypachenko I.V.
Heading	EXPERIMENTAL MEDICINE
Type of article	Scentific article
Annotation	Peripheral nerves injuries by various factors is an actual problem of modern practical and experimental medicine. When inflammatory process affects peripheral nerves, treatment and rehabilitation of such patients takes a long time. The purpose of the study was to establish changes in the morphometric parameters of the sciatic nerve trunk structural elements in rats with a single-dose subcutaneous administration of the cryopreserved placenta. The sciatic nerve trunk was removed from 50 sexually mature rat males of the “Wistar” line. Using a microscope with a digital microphotography, morphometry of the following indices was performed: total thickness of the sciatic nerve, thickness of the epineurium, thickness of the perineurium, thickness of the endoneurium. The trunk of the sciatic nerve consists of an epineurrium that covers the trunk; perineurium - longitudinally oriented collagen (elastic) fibers that act as a membrane; endoneurium, which covers bundles of myelin fibers. It was found that the morphometric parameters of the sciatic nerve trunk grew due to the intercellular space exudation. On the 1st, 2nd, 3rd, and 5th days of the experiment, the total thickness of the sciatic nerve trunk was thickened by 2.8%, 3.4%, 8.2%, 3.5% at (p <0.05); that of endoneurium, according to the terms, - by 2%, 4.1%, 8.7%, 5.1% at (p <0.05). Perineurium and epineurium responded by a significant increase on the 2nd, 3rd and 5th days (p <0.05), by 31,3%, 29,7%, 32,3% and 13,9% 11,7% and 17.8% respectively after a single subcutaneous administration of cryopreserved placenta to rats. In experimental work with complete or partial crossing of the sciatic nerve trunk, against the background of the multipotent stem cells (MSCs) introduction, it was shown that the structural components of the nerve respond by changing their morphometric parameters. Thus, in our studies, it is shown that a single subcutaneous administration of cryopreserved placenta is realized through complex mechanisms of biologically active substances interaction with structural and functional profile of the sciatic nerve, which is manifested by the thickening of the trunk due to the endoneurium edema by 8.7% on the 3rd day, that of epineurium and perineurium - by 17.8% and 32.3% respectively on the 5th day. Rehabilitation of the damaged sciatic nerve is performed by amplifying the apoptosis of damaged cells, increasing the synthesis of cell material against the background of a single-dose subcutaneous administration of the cryopreserved placenta. It may also indicate the presence of neurocytes reparation processes at the early stages after a single subcutaneous administration of the cryopreserved placenta. The above said suggests that the normalization of all morphometric indices of the sciatic nerve on the 7th day may only occur after a single subcutaneous administration of the cryopreserved placenta. It was revealed that the indices of total thickness, epineurium and endoneurium of the sciatic nerve trunk in the rats responded with the index increase, the maximum value being observed on the third day of the experiment and the complete restoration of their parameters on the 7th day. It was found that the restoration of all the indices to the values of the intact group was observed on the 7th day of the experiment. The above facts suggest that the normalization of all morphometric indices of the sciatic nerve for 7 days can only onset after a single-dose administration of the cryopreserved placenta.
Tags	trunk, sciatic nerve, cryopreserved placenta, experiment
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Publication of the article	«World of Medicine and Biology» №4(66), 2018 year, 202-207 pages, index UDK 611.835.8-031:615.368]-07-092.9
DOI	10.26724/2079-8334-2018-4-66-202-207