| About the author: |
O.V. Knysh, O.Yu. Isayenko, O.H. Peretyatko, Ye.M. Babych |
| Heading |
BIOLOGY |
| Type of article |
Scentific article |
| Annotation |
The work is devoted to the study of corynebacteria biofilms formation process under the influence of Bifidobacterium bifidum and Lactobacillus reuteri probiotic strains bacterial derivatives, obtained by the authors-developed method. Five samples of cell-free extracts containing structural components and probiotics metabolites have been studied: filtrates of disintegrates and bifido- and lactobacilli cultures, grown in their own disintegrates. Corynebacteria biofilms were obtained in polystyrene microtiter plates. It has been shown that cell-free derivative-containing extracts have a multidirectional effect on the biofilm formation by corynebacteria at the initial stage, stimulating the biofilm formation by one species of corynebacteria and preventing or inhibiting the biofilm formation by others. Irrespective of the species’ belonging of the corynebacteria, most cell-free extracts inhibit the further growth of the biofilms formed during 24 hours and can cause their degradation. The most pronounced inhibitory effect on biofilm formation is exerted by cell-free extracts obtained from cultures of probiotics grown in their own disintegrates. Anti-biofilm formation activity of the studied cell-free extracts indicates the promising of creating on the basis of probiotics’ derivatives new therapeutic and prophylactic agents for increasing the efficacy of the toxigenic corynebacteria carrier state and diseases caused by opportunistic and pathogenic corynebacteria control. |
| Tags |
corynebacteria, biofilm formation, cell-free extracts, probiotics’ derivatives, Bifidobacterium bifidum, Lactobacillus reuteri |
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| Publication of the article |
«World of Medicine and Biology» №4(66), 2018 year, 235-240 pages, index UDK 579.871.1:579.262:615.372 |
| DOI |
10.26724/2079-8334-2018-4-66-235-240 |
