Palytsia L.M., Korda M.M., Mudra A.E., Fedoniuk L.Y.

FULLERENE C60 STRENGTHEN TOLUENE TOXIC EFFECT ON ENZYMES SYSTEM STATE OF XENOBIOTICS BIOTRANSFORMATION

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About the author:	Palytsia L.M., Korda M.M., Mudra A.E., Fedoniuk L.Y.
Heading	EXPERIMENTAL MEDICINE
Type of article	Scentific article
Annotation	Nanoparticles, due to the wide-scale use in many countries in different areas of production, life and medicine, assume the character of a new global anthropogenic factor, which can be characterized by a potential danger to public health. Preliminary toxicology studies have shown that nanoparticles are characterized by ability to penetrate through biological membranes and physiological barriers of the body and serve as “agents” in the body of heavy metals, pesticides, compounds of halogens, etc. So the question arises about the need for fundamental understanding of toxicological properties of nanoparticles when they penetrate into the body with “classic” chemical toxicants. The aim of this research is to investigate the effect of fullerenes (C60) combination with known chemical toxicant toluene on the activity of the first and second group enzymes of xenobiotics metabolism phase in rat’s liver. Laboratory rats received intraperitoneal injections of fullerene suspension (60 mg/kg), toluene (0.5 ml/kg) or toluene with dissolved fullerenes. UDP-glucuronosyltransferase, ethoxyresorufin-O-deethylase (EROD), glutathione-S-transferase activity were identified in microsomes liver in as a time series starting in hour 3 of the experiment and up to hour 72. Mathematical processing of received data was made in the Department of Statistical Research of I. Horbachevsky Ternopil State Medical University using statistical applications Microsoft Excel 2007 and STATISTICA v6. The results show the average ± SEM from 8 experiments. Got values were compared with the usage of Mann-Witney non-parametric criterion. The changes were considered statistically reliable with р<0,05. Provided that the rats were injected pure suspension of fullerenes С60 in 60 mg/kg dose the indicators of xenobiotics biotransformation didn’t change in comparison with similar indicators of intact animals in any investigation terms. A supposed to fullerenes intraperitoneal injection of toluolin 0,5 ml/kg dose lead to bruskin crease of EROD, microsomes cave liver activity (1/9 times) and UDP-glucuronosyltransferase (1,8 times) in 3 hours after toxicant usage. Enzyme glutathione-S-transferase belongs to the group of II phase xenobiotics detoxification enzyme and participates in toxic substance eradication of different chemical classes very polyaromatic carbohydrate, participates in plants protection from the results of “oxygen explosion” and peroxidises work that activates secondary metabolites of oxidative stress. The activity of this enzyme was decreasing in comparison with the control in 3 and 6 hours after toxin injection (relatively in 1/3 and ½ times). Maximum changes of enzyme system activity functioning indicators of xenobiotics biotransformation is fixed in the animals which were injected by toluol with carbonic nanoparts. Thus the analysis of result stells about out spoken functional changes of enzyme systems of xenobiotics biotransformation I and II phase with chemical toxicant toluol with carbonic nanoparts fullerenes С60 entry into the body. Such synergy of toxic effects of investigated indexes is subjected by fullerenes ability to absorb a big amount of toxin on its surface and helps to transport it to tissues and cells notably to hepatocytes. As the conclusion the carbonic nanoparts fullerenes С60 potentiate the toxic influence of chemical toxicant toluol on xenobiotics biotransformation system.
Tags	fullerenes, toluene, enzymes of xenobiotics biotransformation
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Publication of the article	«World of Medicine and Biology» №1(67), 2019 year, 178-181 pages, index UDK 617.547.533: 546.26-022. 513.2:577.121
DOI	10.26724/2079-8334-2019-1-67-178