| About the author: |
Ananieva M.M., Faustova M.O., Sizova L.M., Reva R.O. |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
In the last decade, the global medical community has noted the growth of infections caused by non-albicans representatives of Candida genus: C.tropicalis, C.parapsilosis, C. saugrata and C.krusei. Among the species represented, the leader in the frequency of occurrence (after C.albicans) is C. saugrata. This causative agent has a generic name, but is phylogenetically different from C.albicans.
In recent years, a large number of studies have been performed on the study of virulence factors C. saugrata and their effect on pathogenesis of the diseases that they cause. Thus, Vale-Silva and Sanglard describe adhesive ability as the main factor of virulence.
The purpose of this study was to investigate the adhesive properties of clinical strains of C.albicans, C.glabrata and the effect of Chlorophylliptum solution on their adhesion process.
Our attention was attracted by the alcohol extract of Eucalyptus globulus, which is produced in Ukraine under the trade name “Chlorophyllipte”. In the instructions for use, the treatment and prophylaxis of Staphylococcus aureus infections is claimed. Currently, this herbal extract is widely used in the world clinical practice as an antibacterial agent.
The study was carries out on 5 clinical isolates of C. albicans and 5 isolates of C.glabrata taken from patients of the Surgery unit. Adhesive potential of the studied clinical strains of microorganisms were evaluated by using the standard method of Brillis V.I. et al. We used an alcoholic solution of Chlorophylliptum thick extract as an active agent and 96% ethanol as an additive (“Halychpharm” corporation, Lviv, Ukraine, No. UA / 4551/02/01 dated October, 31, 2016). The subfungistatic concentrations used in the course of our research were ¼ of fungistatic concentration of the studied extract for each type of pathogen.
C. glabrata can act as an etiologic factor in mucous membrane diseases (oral cavity, esophagus, vagina or urinary tract), as well as severe, life-threatening invasive candidiasis alone or cause mixed infections along with other Candida species, such as C. albicans and C. tropicalis. Therefore, the sensitivity of these microorganisms isolated from patients in the surgical profile to the chlorophylliptide extract and the depression of their adherence under the action of the latter opens up new prospects in the prevention and treatment of fungal postoperative complications.
It was found out that the subfungistatic concentrations of alcohol solution of Chlorophylliptum extract did not affect the adhesive properties of clinical strains of C.albicans. In turn, the Chlorophylliptum extract demonstrated considerable inhibitory effect on the ability of clinical isolates of C. glabrata to adhere to human erythrocytes.
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| Tags |
C.albicans, C. glabrata, adhesiveness, Chlorophylliptum |
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| Publication of the article |
«World of Medicine and Biology» №2(68), 2019 year, 167-170 pages, index UDK 615.322-026.741:616.934 |
| DOI |
10.26724/2079-8334-2019-2-68-167-170 |
