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    Pelypenko O.V., Shepitko V.I., Pelypenko L.B.

    MORPHOFUNCTIONAL CHARACTERISTICS OF A MICROCIRCULATORY BEDS SUBCHONDRAL BONE AT THE INTRODUCTION OF CRYO-CONVERSED PLACENTA ON THE BACKGROUND OF ASEPTIC INFLAMMATION IN THE RAT


    About the author: Pelypenko O.V., Shepitko V.I., Pelypenko L.B.
    Heading EXPERIMENTAL MEDICINE
    Type of article Scentific article
    Annotation The presence of osteoarthritis signs occurs in almost every fifth inhabitant of the globe, which makes this problem very relevant. Despite a significant number of studies on this pathology, the incidence of osteoarthritis in the world is increasing. Attention is drawn to the ambiguity of scientists' views on issues of etiopathogenesis, diagnosis and treatment. In the initiation of degenerative processes of articular cartilage, a significant role is played by the state of the bone vascular system, which provides the functioning of the bone marrow and processes of mineralization of the bone matrix. In comparison with other parts of the tubular bones, subchondral bone parts have a particularly developed vascular network, which results in high metabolic activity of the above site. At the same time, the absence of reserve collateral bypaths leads to a high risk of developing ischemia of the subchondral parts and, accordingly, of trophic disorders around the cartilage sites. Under the conditions of the pathogenic factors impact in the microcirculatory bed, there are phenomena of venous stasis, arterial hypertension, endothelial dysfunction, which ultimately leads to degradation of the cartilage. The purpose of the study was to determine the morphological characteristics of the vascular structures of the subchondral bone of rat knee joints in a comparative aspect in the normal, under the conditions of experimental arthritis and after exposure to preparations of the cryopreserved placenta. An experimental study was carried out on 100 “Wistar” line white male rats, 12 weeks old weighing 130-150 g, which were kept under normal conditions in the vivarium of the Ukrainian Medical Stomatological Academy in compliance with the “Rules for the Use of Laboratory Experimental Animals”, 1984, Annex 4 and with the Helsinki Declaration on the Humane Approach to Animals. The total of 6 animal groups were compared: Group I was intact (10 animals); Group ІІ - intraperitoneal injection of 1 ml of physiological solution - stress reaction (10 animals); Group ІІІ - intraperitoneal injection of 1 ml of physiological solution with additional incision in the area of the outer thigh - repeated stress reaction (10 animals); Group IV - model of aseptic inflammation of the knee joint (30 animals); Group V - subcutaneous implantation of the extract fragment of the placenta (10 animals): Group VІ - subcutaneous implantation of the placenta extract fragment against the background of aseptic inflammation of the knee joint (30 animals). The study of morphological and functional features of the vascular bed of rat subchondral bones in the norm, under the conditions of creating a stressful situation, experimental non-specific arthritis and the introduction of cryopreserved placenta has been carried out. Analysis of vascular bone tissue reaction variants testifies to the efficacy of the cryopreserved placenta used for the prophylaxis and treatment of nonspecific inflammatory processes in the joint.
    Tags microcirculation of bone, cryopreserved placenta, aseptic inflammation
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    Publication of the article «World of Medicine and Biology» №2(68), 2019 year, 187-191 pages, index UDK (612.13:611.71):599.323.4
    DOI 10.26724/2079-8334-2019-2-68-187-191