Pelypenko O.V., Shepitko V.I., Pelypenko L.B.

MORPHOFUNCTIONAL CHARACTERISTICS OF A MICROCIRCULATORY BEDS SUBCHONDRAL BONE AT THE INTRODUCTION OF CRYO-CONVERSED PLACENTA ON THE BACKGROUND OF ASEPTIC INFLAMMATION IN THE RAT

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About the author:	Pelypenko O.V., Shepitko V.I., Pelypenko L.B.
Heading	EXPERIMENTAL MEDICINE
Type of article	Scentific article
Annotation	The presence of osteoarthritis signs occurs in almost every fifth inhabitant of the globe, which makes this problem very relevant. Despite a significant number of studies on this pathology, the incidence of osteoarthritis in the world is increasing. Attention is drawn to the ambiguity of scientists' views on issues of etiopathogenesis, diagnosis and treatment. In the initiation of degenerative processes of articular cartilage, a significant role is played by the state of the bone vascular system, which provides the functioning of the bone marrow and processes of mineralization of the bone matrix. In comparison with other parts of the tubular bones, subchondral bone parts have a particularly developed vascular network, which results in high metabolic activity of the above site. At the same time, the absence of reserve collateral bypaths leads to a high risk of developing ischemia of the subchondral parts and, accordingly, of trophic disorders around the cartilage sites. Under the conditions of the pathogenic factors impact in the microcirculatory bed, there are phenomena of venous stasis, arterial hypertension, endothelial dysfunction, which ultimately leads to degradation of the cartilage. The purpose of the study was to determine the morphological characteristics of the vascular structures of the subchondral bone of rat knee joints in a comparative aspect in the normal, under the conditions of experimental arthritis and after exposure to preparations of the cryopreserved placenta. An experimental study was carried out on 100 “Wistar” line white male rats, 12 weeks old weighing 130-150 g, which were kept under normal conditions in the vivarium of the Ukrainian Medical Stomatological Academy in compliance with the “Rules for the Use of Laboratory Experimental Animals”, 1984, Annex 4 and with the Helsinki Declaration on the Humane Approach to Animals. The total of 6 animal groups were compared: Group I was intact (10 animals); Group ІІ - intraperitoneal injection of 1 ml of physiological solution - stress reaction (10 animals); Group ІІІ - intraperitoneal injection of 1 ml of physiological solution with additional incision in the area of the outer thigh - repeated stress reaction (10 animals); Group IV - model of aseptic inflammation of the knee joint (30 animals); Group V - subcutaneous implantation of the extract fragment of the placenta (10 animals): Group VІ - subcutaneous implantation of the placenta extract fragment against the background of aseptic inflammation of the knee joint (30 animals). The study of morphological and functional features of the vascular bed of rat subchondral bones in the norm, under the conditions of creating a stressful situation, experimental non-specific arthritis and the introduction of cryopreserved placenta has been carried out. Analysis of vascular bone tissue reaction variants testifies to the efficacy of the cryopreserved placenta used for the prophylaxis and treatment of nonspecific inflammatory processes in the joint.
Tags	microcirculation of bone, cryopreserved placenta, aseptic inflammation
Bibliography	Elkerton JS., Xu Y., Pickering JG, Ward AD. Differentiation of arterioles from venules in mouse histology images using machine learning. J. Med. Imag. 2017 Apr-Jun; 4(2): 021104(1-10). Felson DT1, McLaughlin S, Goggins J, LaValley MP, Gale ME, Totterman S, Li W, Hill C, Gale D. Bone marrow edema and its relation to progression of knee osteoarthritis. Ann Intern Med. 2003 Sep 2;139(5 Pt 1):330-6. Finnila MA., Thevenot J., Aho OM., Tiitu V., Rautiainen J., Kauppinen S., et al. Association between subchondral bone structure and osteoarthritis histopathological grade. J Orthop Res. 2017Apr; 35(4):785-792. Golovach IY. Osteoarthritis: fundamental and applied aspects of the disease’s etiopathogenesis. nothing ever stands still. Ukrainian journal of rheumatology. 2014; 2(56):4-11. Kabalyk MA. The role of vascular factors in the pathogenesis of osteoarthritis. Modern problems of science and education. 2017; 2:50. Murylev VY, Sorokina GL, Kurilina EV, Ivanenko LR. Subchondral bone condition and total knee arthroplasty in patients with knee osteoarthritis. Osteoporosis and bone diseases. 2017; 20(1):12-16. Pelipenko AV., Shepitko VI, Quantitative relationships of the structural components of bone tissue of rats. Bulletin of problems biology and medicine. 2016; 2(2):249–52. Shirinsky VS., Kazygasheva EV., Shirinsky IV. Inflammation and immunity: a role of pathogenesis of osteoarthritis. Medical Immunology (Russia). 2019; 21(1):39-48. Sviridenko AS., Bunov VS., Biryukova MY., Lyulin SV., Mescheryagina IA. Features of a circulation in the femoral condyle and in the tibia in a 2-nd degree gonarthrosis. Iinternational journal of applied and fundamental research. 2016; 3(1):94-9. Yakovenchuk NN., Dedukh NV. Morphology of joint cartilage and subhondral bone plate after modeling osteoporosis. Bulletin of problems in biology and medicine. 2017; 4 (3 (141)):324-7.
Publication of the article	«World of Medicine and Biology» №2(68), 2019 year, 187-191 pages, index UDK (612.13:611.71):599.323.4
DOI	10.26724/2079-8334-2019-2-68-187-191