Shatorna V.F., Harets V.I., Nefodova O.O., Halperin O.I., Deforzh G.V., Gruzd V.V.

EXPERIMENTAL DETERMINATION OF THE INFLUENCE OF CITRATES OF METALS TO EMBRYOTOXICITY OF CADIUM SALTS IN EMBRYOGENESIS OF RATS

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About the author:	Shatorna V.F., Harets V.I., Nefodova O.O., Halperin O.I., Deforzh G.V., Gruzd V.V.
Heading	EXPERIMENTAL MEDICINE
Type of article	Scentific article
Annotation	Until now, despite a large number of morphological studies devoted to the study of the influence of heavy metals to the development of the heart during ontogenesis, there remain a number of unresolved issues regarding the understanding of changes in the main morphogenetic events of embryo development and cardiogenesis. The results of numerous studies confirm that one of the etiopathogenetic causes is the influence of environmental factors, namely cadmium salts. Direct observation of the development of human developmental disorders is not possible, therefore, with the help of induced experimental models it becomes possible to analyze morphogenetic changes during embryogenesis. Thus, the actual direction of morphological experimental studies is the detection of a spectrum of violations of the general course of embryogenesis under the influence of cadmium salts on pregnant females and under conditions of compensation of citrates of metals. The purpose of the study is to experimentally determine the degree of accumulation of cadmium in the body of embryos and the effect on the overall course of embryogenesis of cadmium salts when administered in isolation and in combination with metal citrates in rats. Materials and methods of research. Experimental studies were performed on female Wistar rats (Nursery "Dali", Kyiv). To simulate the effects and toxic effects of exposure to cadmium salts, during the entire period of pregnancy, female rats of the Wistar line enter the solutions of the substances under study every day. All rats were divided into 8 groups: 1 group - control. Group 2 - Animals administered a solution of cadmium chloride at a dose of 1.0 mg / kg. Group 3 - Animals administered a 1.0 mg / kg solution of cadmium citrate. Group 4 - Animals administered a 1.0 mg / kg solution of cadmium chloride and a cerium citrate solution at a dose of 1.3 mg / kg. Group 5 - Animals administered a 1.0 mg / kg solution of cadmium citrate and a cerium citrate solution at a dose of 1.3 mg / kg. Group 6 - Animals administered a 1.0 mg / kg solution of cadmium chloride and a solution of germanium citrate in a dose of 0.1 mg / kg. Group 7 - Animals administered a 1.0 mg / kg solution of cadmium citrate and a solution of germanium citrate in a dose of 0.1 mg / kg. Group 8 - Animals administered a solution of cadmium chloride at a dose of 1.0 mg / kg and a solution of sulfur and iodine citrates in a dose of 0.003 mg / kg. The results of the experiment revealed a more pronounced embryotoxic effect of cadmium chloride compared to the effects of cadmium citrate at their same dose and route of administration in an experiment on rats. Combined administration of cadmium chloride / citrate with cerium / germanium / iodine + sulfur citrates significantly reduces the embryotoxic effect of cadmium, which is manifested in the increase in the number of embryos in the litter and the decrease in both general and preimplantation and post implantational embryonic mortality rates at the 13th and 20th days of embryogenesis of rat. Results of measurements of cadmium content in the heart of embryos indicate a decrease in the cadmium content in the body of embryos when combined with the introduction of cadmium salts with cerium citrate / germanium citrate / iodine + sulfur citrate upon intragastric administration in experiments on rats and these elements can be considered as new cadmium bioanagonists.
Tags	embryogenesis, cadmium, cerium, germanium, iodine, sulfur
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Publication of the article	«World of Medicine and Biology» №2(68), 2019 year, 210-214 pages, index UDK 611.12-076:611.013:616-092.9:669.018.674
DOI	10.26724/2079-8334-2019-2-68-210-214