| About the author: |
Tverdokhlib I.V., Marchenko D.G. |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
Information on the formation of the contractile apparatus of the heart and the distribution of myofibrils in cardiomyocytes under conditions of intrauterine intoxication with ethanol remain a subject of considerable debate. The aim of the study was to determine changes in the ultrastructure of the contractile apparatus of rat ventricular cardiomyocytes during prenatal development in conditions of intrauterine alcohol intoxication. It was determined that chronic alcohol intoxication in prenatal cardiogenesis damages the contractile apparatus of ventricular cardiomyocytes due to disorganization of the structure of sarcomeres, fragmentation and disorientation of myofibrils, significant inhibition of sarcomere genesis, and a decrease in the content of myofibrils, which is associated with destruction of mitochondria. The severity of changes in these structures depends on the zone and period of development of the embryo. The most significant changes are due to the direct toxic effect of ethanol and occur in the early stages of cardiogenesis. |
| Tags |
prenatal ontogenesis, alcohol intoxication, ventricular myocardium, myofibrils, ultrastructure |
| Bibliography |
- Avtandilov GG. Meditsinskaya morfometriya: rukovodstvo [Medical morphometry: guideline]. Moscow: Meditsina; 1990. 384 p. [in Russian]
- Ivanchenko MV, Tverdokhlib IV. Formuvannya mitokhondrialnoho aparata skorotlyvykh kardiomiotsytiv v normi ta za umov hipoksychnoho ushkodzhennya kardiohenezu. Morphologia. 2013;7(1):5-20. [in Ukrainian]
- Tverdokhlib IV, Petruk NS, Ivanchenko MV, Silkina JuV, Khripkov IS, Pertseva NO, Shevchenko KM, Goodlett TO, Malkov II, Berehovenko IM, Zinenko DYu, Galaida NO, Varin VV, inventors; State Institution «Dnipropetrovsk medical academy of the Health Ministry of Ukraine». Method of determining the coordinates of ultrastructures in transmission electron microscopy of biological objects. Ukrainian patent UA 83611. 2013 Sep 25. Int. CI. G01N 1/28. [in Ukrainian]
- Conen D. Alcohol consumption and incident cardiovascular disease: not just one unifying hypothesis. Eur. Heart J. 2015;36(15):897–8.
- Drew NK, Grosberg A. Methods of myofibrillogenesis modeling. Methods Mol. Biol. 2015;1299:75–91.
- European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Strasbourg: Council of Europe. 18 Mar 1986. 53 p.
- Fenix AM, Neininger AC, Taneja N, Hyde K, Visetsouk MR, Garde RJ, Liu B, Nixon BR, Manalo AE, Becker JR. Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes. Elife. 2018 Dec 12;7. Epub 2018 Dec 12.
- Gardner JD, Mouton AJ. Alcohol effects on cardiac function. Compr. Physiol. 2015;5(2):791-802.
- Geach TJ, Hirst EM, Zimmerman LB. Contractile activity is required for Z-disc sarcomere maturation in vivo. Genesis. 2015;53(5):299-307.
- Hayat МА. Principles and techniques of electron microscopy: Biological applications [4th ed.]. Cambridge : Cambridge University Press; 2000. 543 p.
- Kamran K, Khan MY, Minhas LA. Atrial and ventricular septal changes in ethanol vapour exposed chick embryos. J. Pak. Med. Assoc. 2015;65(3):296–9.
- Sanger JW, Wang J, Fan Y, White J, Mi-Mi L, Dube DK, Sanger JM, Pruyne D. Assembly and Maintenance of Myofibrils in Striated Muscle. Handb Exp Pharmacol. 2017;235:39-75.
- Wang J, Fan Y, Sanger JM, Sanger JW. Nonmuscle myosin II in cardiac and skeletal muscle cells. Cytoskeleton (Hoboken). 2018;75(8):339-51.
- Zile MR, Baicu CF, Ikonomidis JS. Myocardial stiffness in patients with heart failure and a preserved ejection fraction: contributions of collagen and titin. Circulation. 2015;131(4):1247-59.
|
| Publication of the article |
«World of Medicine and Biology» №3(69), 2019 year, 225-230 pages, index UDK 611.12:611.018:611.013:576.311.348.3 |
| DOI |
10.26724/2079-8334-2019-3-69-225-230 |
