| About the author: |
Shkolnikov V.S., Prykhodko S.O., Polishchuk S.S., Kryvoviaz O.V., Galunko G.М. |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
The study of radial glia morphology and processes of targeted migration of neural stem cells in humans remains far from being resolved. The purpose of this study is to establish the morphological aspects of radial glia of spinal cord of human in prenatal period. Morphological examination of the spinal cord of human embryos and fetuses of 6-7 weeks up to 39-40 weeks was performed. Using anatomical, histological, immunohistochemical, and morphometric morphological aspects of the radial glia of the spinal cord were established. The results showed that strong expression of vimentin and CDX-2 radial glia fibers was observed up to 8-9 weeks. From 11-12 weeks, radial glia fibers retain a radial direction only in the middle part of the segments, which, in our view, is associated with the gradual involution of radial glia, which correlates with the formation of nuclear-neural complexes of gray matter. Until the moment of birth, vimentin-positive structures of radial glia gradually disappear and can be traced only in the neuroepithelium of segments. |
| Tags |
prenatal period, spinal cord, radial glia, neuroepitelium, immunohistochemical markers |
| Bibliography |
- Kirik OV, KorzhevskiiDE. Vimentin v kletkah ependimy I subventricularnoy proliferativnoy zony konechnogo mozga. Kletochnyye technologii v biologii і meditsine. 2012; 4:210-4. [in Russian]
- Lubych LD, Lisyanyi MI. Imunobiologichni vlastyvosti neirohennykh klityn fetalnoho mozku. Fiziolohichnyi Zhurnal. 2017; 63(6):118-35. [in Ukrainian]
- Rybachuk OA, Pivneva TA. Rol neiralnykh stovburovykh klityn v reheneratsii tsentralnoyi nervovoyi systemy. Fiziolohichnyi Zhurnal. 2013; 59(2):111-121. [in Ukrainian]
- Shkolnikov VS. Osoblyvosti histohenesu matrychnoho sharu spynnoho mozku embrioniv ta plodiv lyudyny. Lvivskyi medychnyi chasopys. 2015; 1:29-34. [in Ukrainian]
- Tsymbaluk VI, Medvedev VV. Spinnoy mozg. Elegiya nadezhdy. Vinnitsa: Nova knyga; 2010. 944 s. [in Russian]
- Fedorkovskaya BO. Morfofunkcionalnyye osobennosti razvitiya notsitseptivnoy sistemy u novorozhdennykh. Meditsina neotlozhnykh sostoyaniy. 2013; 3:4-7. [in Russian]
- Barry DS, Pakan JM, O'Keeffe GW, McDermott KW. The spatial and temporal arrangement of the radial glial scaffold suggests a role in axon tract formation in the developing spinal cord. Journal of Anatomy. 2013; 222(2):203-13.
- Barry DS, Pakan JM, McDermott KW. Radial glial cells: key organizers in CNS development. The International Journal of Biochemistry & Cell Biology. 2014; 46:76-9.
- Costa V, Lugert S, Jagasia R. Role of adult hypocampal neurogenesis in cognition in physiology and disease: pharmacological targets and biomarkers. Handbook of Experimental Pharmacology. 2015; 228:99-155.
- Harris L, Zalucki O, Piper M, Heng JI. Insights into the Biology and Therapeutic Applications of Neural Stem Cells. Stem Cells Int [serial on the Internet]. 2016 Mar [cited 2017 Sep 8]; 2016: 9745315 [18p.].
- Montalban-Loro R, Domingo-Muelas A, Bizy A. Epigenetic regulation of stemness maintenance in the neurogenic niches. World Journal Stem Cells. 2015; 7(4):700-10.
- Nowakowski T, Pollen A, Sandoval-Espinosa С, Kriegstein А. Transformation of the radial glia scaffold demarcates two stages of human cerebral cortex development. Neuron. 2016; 91(6):1219-27.
- Ostrem B, Lui J, Gertz C, Kriegstein A. Control of outer radial glial stem cell mitosis in the human brain. Cell Report. 2014; 8(3):656-64.
- Rueger MA, Schroeter M. In vivo imaging of endogenous neural stem cells in the adult brain. World Journal Stem Cells. 2015; 7(1):75-83.
- Vishwakarma SK, Bardia A, Tiwari SC, Paspala SA, Khan AA. Current concept in neural regeneration research: NSCs isolation, characterization and transplantacion, in various neurodegenerative diseases and stroke: A review. Journal of Advanced Research. 2014; 5(3):277-94.
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| Publication of the article |
«World of Medicine and Biology» №2(72), 2020 year, 229-234 pages, index UDK 572.7:612.83:57.017.642 |
| DOI |
10.26724/2079-8334-2020-2-72-229-234 |
