| About the author: |
Т. P. Pirog, І. V. Kliuchka, D. А. Lutsai, L. V. Kliuchka, O. I. Skrotska |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
It was found that the minimum inhibitory concentrations against bacteria and yeast of Acinetobacter calcoaceticus IMV B-7241, Rhodococcus erythropolis IMV Ac-5017 and Nocardia vaccinii IMV B-7405 surfactants, synthesized on traditional substrates, were 9–120 μg/ml and were within the limits defined for the surfactants known in the world. It was for the first time established that surfactants synthesized by the study strains on wastes of biodiesel production and fried sunflower oil were characterized by high antimicrobial activity against bacteria and yeast (minimum inhibitory concentrations 0.45–120 and 1.9–142 μg/ml respectively). It was found that the added of both live and inactivated Escherichia coli ІEM-1 and Bacillus subtilis BT-2 cells in R. erythropolis IMV Ac-5017 and N. vaccinii IMV B-7405 medium cultivation was accompanied by synthesis of surfactants, minimum inhibitory concentrations of which were several times lower than those showed for surfactants synthesized without competitive microorganisms. The obtained results indicate the possibility of using the studied surfactants as effective antimicrobial agents. |
| Tags |
microbial metabolites, antimicrobial agents, minimum inhibitory concentrations, wastes of biodiesel production, fried sunflower oil, competitive microorganisms |
| Bibliography |
- Aleksic I, Petkovic M, Jovanovic M,Milivojevic D, Vasiljevic B, Nikodinovic-Runic J, et al. Anti-biofilm properties of bacterial di-rhamnolipids and their semi-synthetic amide derivatives. Front Microbiol. 2017; 8:2454. doi: 10.3389/fmicb.2017.02454.
- Aslam B, Wang W, Arshad MI, Khurshid M, Muzammil S, Rasool MH et al. Antibiotic resistance: a rundown of a global crisis. Infect Drug Resist. 2018; 11:1645−1658. doi: 10.2147/IDR.S173867.
- Balan SS, Kumar CG, Jayalakshmi S. Aneurinifactin, a new lipopeptide biosurfactant produced by a marine Aneurinibacillus aneurinilyticus SBP-11 isolated from Gulf of Mannar: Purification, characterization and its biological evaluation. Microbiol. Res. 2017; 194:1−9. doi: 10.1016/j.micres.2016.10.005.
- Benitez L, Correa A, Daroit D, Brandelli A. Antimicrobial activity of Bacillus amyloliquefaciens LBM 5006 is enhanced in the presence of Escherichia coli.Curr Microbiol. 2011; 62(3):1017−1022. doi: 10.1007/s00284-010-9814-z.
- Cattoir V., Felden B. Future antibacterial strategies: from basic concepts to clinical challenges. J. Infect. Dis. 2019. pii: jiz134. doi: 10.1093/infdis/jiz134.
- Chebbi A, Elshikh M, Haque F. Ahmed S, Dobbin S, Marchant R, et al. Rhamnolipids from Pseudomonas aeruginosa strain W10; as antibiofilm/antibiofouling products for metal protection. J Basic Microbiol. 2017; 57(5): 364−375. doi: 10.1002/jobm.201600658.
- Dalili D, Amini M, Faramarzi MA. Fazeli MR, Khoshayand MR, Samadi N. Isolation and structural characterization of Coryxin, a novel cyclic lipopeptide from Corynebacterium xerosis NS5 having emulsifying and anti-biofilm activity. Colloids Surf. B Biointerfaces. 2015; 135: 425-432. doi:10.1016/j.colsurfb.2015.07.005.
- Founou R.C., Founou L.L., Essack S.Y. Clinical and economic impact of antibiotic resistance in developing countries: systematic review and meta-analysis. PLoS One. 2017, 12(12):e0189621. doi: 10.1371/journal.pone.0189621.
- Huang E, Yousef AE. Paenibacterin, a novel broad-spectrum lipopeptide antibiotic, neutralises endotoxins and promotes survival in a murine model of Pseudomonas aeruginosa-induced sepsis. Int J Antimicrob Agents. 2014; 44(1): 74−77. doi:10.1016/j.ijantimicag.2014.02.018.
- Li B, Li Q, Xu Z, Zhang N, Shen Q, Zhang R. Responses of beneficial Bacillusamyloliquefaciens SQR9 to different soilborne fungal pathogens through the alteration of antifungal compounds production. Front Microbiol. 2014; 5. doi: 10.3389/fmicb.2014.00636.
- Ndlovu T, Rautenbach M, Vosloo JA, Khan S, Khan W. Characterisation and antimicrobial activity of biosurfactant extracts produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa isolated from a wastewater treatment plant. AMB Express. 2017; 7(1):108. doi: 10.1186/s13568-017-0363-8.
- Pirog T., Sofilkanych A., Konon A., Shevchuk T., Ivanov S. Intensification of surfactants’ synthesis by Rhodococcus erythropolis IMV Ac-5017, Acinetobacter calcoaceticus IMV B-7241 and Nocardia vaccinii K-8 on fried oil and glycerol containing medium. Food Bioprod Process. 2013; 91(2): 149−157. http://dx.doi.org/10.1016/j.fbp.2013.01.001.
- Pirog TP, Sofilkanych AP, Shulyakova M. Shevchuk TA. Biosurfactant synthesis by Rhodococcus erythropolisIMV Ac-5017, Acinetobacter calcoaceticus IMV B-7241 and Nocardia vaccinii IMV B-7405 on byproduct of biodiesel production. Food Bioprod Process. 2015; 93: 11–18. https://doi.org/10.1016/j.fbp.2013.09.003.
- Tazdaït D, Salah R, Mouffok S.Preliminary evaluation of a new low-cost substrate (amurca) in production of biosurfactant by Pseudomonas aeruginosa isolated from fuel-contaminated soil. J. Mater. Environ. Sci. 2018; 9(3):964−970.
- Vollbrecht E, Rau U, Lang S. Microbial conversion of vegetable oils into surfaceactive di-, tri-, and tetrasaccharide lipids (biosurfactants) by the bacterial strain Tsukamurella spec. Lipid Fett. 1999; 101(10): 389−394.
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| Publication of the article |
«World of Medicine and Biology» №4(78), 2021 year, 237-241 pages, index UDK 579.663 |
| DOI |
10.26724/2079-8334-2021-4-78-237-241 |
