| About the author: |
N. Ya. Kravets, S. I. Klumnyk, L. B. Romanyuk, V. P. Borak |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
Among the causes of chronic infection and resistance to antibiotic treatment are the formation of biofilms in the respiratory tract in respiratory infections. The purpose of the study is to identify the ability to film strains of S. aureus on the surface of the epithelial tissue of the oropharynx isolated from patients with recurrent tonsillitis. Clinical strains of Staphylococcus spp bacteria obtained from the oropharynx of 42 children with recurrent tonsillitis were investigated. The ability to form biofilms – when grown on 96-well plastic plates and on the surface of coverslips for three days. It was found that 12 (28.58 %) strains out of 42 had film-forming ability, among which 3 (7.14 %) strains showed strong film-forming, 8 (19.04 %) strains were moderate, one strain (2.38 %) – a moderate formation film and 30 strains (71.4 %) lacked this ability. The severity of staphylococcus film formation was determined by optical density at a wavelength of 550 nm. Conducted studies of the intensity of film formation over three days of cultivation, the rate of OD was 0.054
|
| Tags |
biofilm, recurrent tonsillitis, degree of biofilm formation, Staphylococcus spp |
| Bibliography |
- Abu Bakar M, McKimm J, Haque SZ, Majumder MAA, Haque M. Chronic tonsillitis and biofilms: a brief overview of treatment modalities. J Inflamm Res. 2018 Sep 5;11:329-337. doi: 10.2147/JIR.S162486
- Azeredo J, Azevedo NF, Briandet R, Cerca N, Coenye T, Costa AR, et al. Critical review on biofilm methods. Crit Rev Microbiol. 2017 May;43(3):313-351. doi: 10.1080/1040841X.2016.1208146 205677
- Bulut F, Meric F, Yorgancilar E, Nergiz Y, Akkus M, Nergiz S, Nasir Y. Effects of N-acetyl-cysteine and acetylsalicylic acid on the tonsil bacterial biofilm tissues by light and electron microscopy Eur Rev Med Pharmacol Sci. 2014; 18 (23): 3720–3725 PMID: 25535147.
- Cai YM, Zhang YD, Yang L. NO donors and NO delivery methods for controlling biofilms in chronic lung infections. Appl Microbiol Biotechnol 2021;105:3931–3954. doi:10.1007/s00253-021-11274-2
- Kravets N, Klymnyuk S, Romanyuk L, Borak V, Mykhailyshyn H. Investigation of strains of microorganisms isolated from children with recurrent tonsillitis. Georgian Med News. 2020; 298:49–53.
- Le KY, Villaruz AE, Zheng Y, He L, Fisher EL, Nguyen TH, et al. Role of Phenol-Soluble Modulins in Staphylococcus epidermidis Biofilm Formation and Infection of Indwelling Medical Devices. J Mol Biol. 2019 Jul 26;431(16):3015-3027. doi: 10.1016/j.jmb.2019.03.030. Epub 2019 Apr 5. PMID: 30954574.
- Lynch C, O'Connor JA, O'Brien D, Vaughan C, Bolton D, Coffey A, et al. First reported detection of biofilm formation by Campylobacter fetus during investigation of a case of prosthetic valve endocarditis. J Clin Pathol. 2019 Aug;72(8):554-557. doi: 10.1136/jclinpath-2018-205677
- Mani S, Rekha A, Srinivasan MKR, Bhanumathy V. To Evaluate the Role of H. pylori in Patients with Chronic Recurrent Tonsillitis. Indian J Otolaryngol Head Neck Surg. 2019 Jun;71(2):254-258. doi: 10.1007/s12070-018-1313-8.
- Marchenko AV, Loban GA, Petrushanko TО, Chereda VV, Faustova MO, Ananieva MM. The effect of the psycho-emotional stress on the state of microbiota of the gingival sulcus. Svit medytsyny ta biolohiyi. 020; 3(73): 69–74. doi: 10.26724/2079-8334-2020-3-73-69-74
- Nazzari E, Torretta S, Pignataro L, Marchisio P, Esposito S.. Role of biofilm in children with recurrent upper respiratory tract infections. Eur J Clin Microbiol Infect Dis. 2015; 34(3): 421–429. doi: 10.1007/s10096-014-2261-1
- Philip N, Leishman SJ, Bandara H, Walsh LJ. Polyphenol-Rich Cranberry Extracts Modulate Virulence of Streptococcus mutans-Candida albicans Biofilms Implicated in the Pathogenesis of Early Childhood Caries. Pediatr Dent. 2019 Jan 15;41(1):56-62. PMID: 30803479.
- Saafan ME. Endoscopic management of congenital bilateral posterior choanal atresia: value of using stents. Eur Arch Otorhinolaryngol. 2013; 270(1): 129–134. doi: 10.1007/s00405-012-1956-0
- Torretta S, Drago L, Marchisio P, Cappadona M, Rinaldi V, Nazzari E, et al. Recurrences in chronic tonsillitis substained by tonsillar biofilm-producing bacteria in children. Relationship with the grade of tonsillar hyperplasy. Int J Pediatr Otorhinolaryngol. 2013 Feb;77(2):200-4. doi: 10.1016/j.ijporl.2012.10.018. Epub 2012 Nov 5. PMID: 23137856.
- Yousefi M, Pourmand MR, Fallah F, Hashemi A, Mashhadi R, Nazari-Alam A. Characterization of Staphylococcus aureus Biofilm Formation in Urinary Tract Infection. Iran J Public Health. 2016;45(4):485-493.
|
| Publication of the article |
«World of Medicine and Biology» №2(80), 2022 year, 210-213 pages, index UDK 616.322-002.2:576.851.252-053.2-074 |
| DOI |
10.26724/2079-8334-2022-2-80-210-213 |
