| About the author: |
A. V. Kuraieva, S. I. Savosko, A. N. Grabovyi, O. M. Makarenko |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
Astrogliosis is a typical astrocyte response to intracerebral hemorrhage. The purpose of the study was to analyze morphological changes in astrocytes in the cerebral cortex, hippocampus, and striatum of rats with intracerebral hemorrhage and after administration of dexamethasone, granulocyte colony-stimulating factor and their combination. Astrocyte morphology, changes in projection perimeter and their density on days 1, 3, 10, 30 and 60 in three brain regions were studied based on the results of immunohistochemical staining for glial fibrillary acidic protein in astrocytes. Dexamethasone stimulated astrocyte hypertrophy in the cerebral cortex on days 3 and 10, and the granulocyte colony-stimulating factor caused an increase in astrocyte density on day 3 of the study. The morphological features of astrocyte response in the study regions of the brain were different: active hypertrophy and gliosis in the striatum, and predominantly hypertrophy of processes in the cerebral cortex and hippocampus. |
| Tags |
intracerebral hemorrhage,astrocytes,dexamethasone,granulocyte colony-stimulating factor |
| Bibliography |
- Grabovyi OM, Nevmerzhytska NM, Yaremenko LM, Kostynskyi HB, Shepelev SIe, Demedchuk AS, Bidna LP. Ekspresiya CD44 u klitynakh reheneratsiynoyi nevromy sidnychoho nervu za umov diyi hranulotsytarnoho koloniye-stymuliuyuchoho faktoru. Materialy naukovo-praktychnoyi konferentsiyi Basic Medical Science for Endocrinology (17-18 lystopada 2022 m. Ivano-Frankivsk). р. 22–3. [in Ukrainian]
- Begum G, Song S, Wang S, Zhao H, Bhuiyan MIH, Li E, et al. Selective knockout of astrocytic Na(+) /H(+) exchanger isoform 1 reduces astrogliosis, BBB damage, infarction, and improves neurological function after ischemic stroke. Glia. 2018; 66:126-44. doi: 10.1002/glia.23232.
- Chun H, Lim J, Park KD, Lee CJ. Inhibition of monoamine oxidase B prevents reactive astrogliosis and scar formation in stab wound injury model. Glia. 2022;70(2):354–67. doi: 10.1002/glia.24110.
- Ding ZB, Song LJ, Wang Q, Kumar G, Yan YQ, Ma CG. Astrocytes: a double-edged sword in neurodegenerative diseases. Neural Regen Res. 2021;16(9):1702–10. doi: 10.4103/1673-5374.306064.
- Gumenyuk AV, Tykhomyrov AA, Savosko SI, Guzyk MM, Rybalko SL, Ryzha АО, Chaikovsky YuB. State of Astrocytes in the Mice Brain under Conditions of Herpes Viral Infection and Modeled Stroke. Neurophysiology. 2018;50(5):326–31. doi: https://doi.org/10.1007/s11062-019-09757-0.
- Klemens J, Ciurkiewicz M, Chludzinski E, Iseringhausen M, Klotz D, Pfankuche VM, et al. Neurotoxic potential of reactive astrocytes in canine distemper demyelinating leukoencephalitis. 2019; 9:11689. Doi: https://doi.org/10.1038/s41598-019-48146-9.
- Li L, Zhou J, Han L, Wu X, Shi Y, Cui W, et al. The Specific Role of Reactive Astrocytes in Stroke. Front Cell Neurosci. 2022; 16:850866. Doi: 10.3389/fncel.2022.850866.
- Martynenko RV, Shepitko VI, Stetsuk YeV, Boruta NV, Yakushko OS, Martynenko VB. Complex histological and immunohistochemical study of monocytopoiesis in rats with determination of proliferative activity by expression ki67 and cd68+ in the experiment. World of Medicine and Biology. 2021;4(78):229–32. Doi: 10.26724/2079-8334-2021-4-78-229-232.
- Merkel SF, Andrews AM, Lutton EM, Razmpour R, Cannella LA, Ramirez SH. Dexamethasone Attenuates the Enhanced Rewarding Effects of Cocaine Following Experimental Traumatic Brain Injury. Cell Transplant. 2017;26(7):1178–92. Doi: 10.1177/0963689717714341.
- Neves JD, Mestriner RG, Netto CA. Astrocytes in the cerebral cortex play a role in the spontaneous motor recovery following experimental striatal hemorrhage. Neural Regen Res. 2018;13(1):67–8. Doi: 10.4103/1673-5374.224372.
- Ren Y, Ao Y, O’Shea TM, Burda JE, Bernstein AM, Brumm AJ, et al. Ependymal cell contribution to scar formation after spinal cord injury is minimal, local and dependent on direct ependymal injury. Sci. Rep. 2017; 7:41122. Doi: 10.1038/srep41122.
- Vizuete AFK, Hansen F, Negri E, Leite MC, de Oliveira DL, Gonçalves CA. Effects of dexamethasone on the Li-pilocarpine model of epilepsy: protection against hippocampal inflammation and astrogliosis. J Neuroinflammation. 2018;15(1):68. Doi: 10.1186/s12974-018-1109-5.
- Xiong L, Yang Y, Zhang M, Xu W. The use of serum glial fibrillary acidic protein test as a promising tool for intracerebral hemorrhage diagnosis in Chinese patients and prediction of the short-term functional outcomes. Neurol Sci. 2015;36(11):2081–7. Doi: 10.1007/s10072-015-2317-8.
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| Publication of the article |
«World of Medicine and Biology» №4(86), 2023 year, 206-211 pages, index UDK 616.8:616.009:616-092:591.4 |
| DOI |
10.26724/2079-8334-2023-4-86-206-211 |
