FEATURES OF BIOCHEMICAL MARKERS OF RAT BLOOD SERUM CAUSED BY MODELING OF PEROXIDATIVE PERIODONTITIS, ALIMENTARY PROTEIN DEFICIENCY BY MEANS OF A THERAPEUTIC AND PROPHYLACTIC COMPLEX

Authors:

Abstract:

The study was devoted to the researching of the effect of the therapeutic and prophylactic complex on the blood serum biochemical markers in rats with peroxidative periodontitis and protein deficiency. The experiment involved 30 male Wistar rats divided into three groups: intact, a group with combined pathology and a group with combined pathology, which received the therapeutic and prophylactic complex. After 60 days, total bilirubin, total cholesterol, total protein and elastase activity were quantified in serum. Combined pathology elevated bilirubin, depleted total protein and increased elastase activity versus controls, confirming hepatobiliary stress, hypoproteinaemia and inflammation. Therapeutic and prophylactic complex normalised bilirubin, restored protein and reduced elastase compared with untreated pathology, while lowering cholesterol beneath intact values. The data highlight the hepatoprotective, protein-sparing, lipid-modulating and anti-inflammatory potential of the complex.

Keywords:

periodontitis blood serum rats experiment biochemical markers

References:

1. Hubskyy YuI, Nizhenkovska IV, Korda MM. Biolohichna i bioorhanichna khimiya: u 2 kn.: pidruchnyk. Kn.2. Biolohichna khimiya. Kyiv: VSV “Medytsyna”, 2016. 497–506. [in Ukrainian].
2. Makarenko OA, Khromahina LM, Khodakov IV. Metody doslidzhennya stanu kyshkovyka ta kistok u laboratornykh shchuriv: dovidnyk. Odesa: Odeskyy natsionalnyy universytet im. I.I. Mechnykova, 2022. 81 p. [in Ukrainian].
3. Nakaz Ukrayiny “Pro zatverdzhennya Poryadku provedennya naukovymy ustanovamy doslidiv, eksperymentiv na tvarynakh”. Ministerstvo osvity i nauky Ukrayiny. 2012;249. [in Ukrainian].
4. Rohach IM, Keretsman AO, Sitkar AD. Pravylno vybranyy metod statystychnoho analizu – shlyakh do yakisnoyi interpretatsiyi danykh medychnykh doslidzhen. Naukovyy visnyk Uzhhorodskoho universytetu, seriya “Medytsyna”. 2017;2(56):124–128. [in Ukrainian].
5. Savvova OV, Voronov HK, Fesenko OI, Smyrnova YuO. Bioaktyvni materialy dlya reheneratsiyi kistkovoyi tkanyny: navch. posibnyk. Kharkiv: KHNUMH im. O. M. Beketova, 2021. 142 p. [in Ukrainian].
6. Benahmed AG, Tippairote T, Gasmi A, Noor S, Avdeev O, Shanaida Y, et al. Periodontitis Continuum: Antecedents, Triggers, Mediators, and Treatment Strategies. Curr Med Chem. 2024;31(41):6775–6800. DOI: 10.2174/0109298673265862231020051338.
7. European convention for the protection of vertebrale animals used for experimental and other scientific purposes. Strasburg.Council of Europe. 1986;123:51.
8. Jiang Y, Song B, Brandt BW, Cheng L, Zhou X, Exterkate RAM, et al. Comparison of Red-Complex Bacteria Between Saliva and Subgingival Plaque of Periodontitis Patients: A Systematic Review and Meta-Analysis. Front Cell Infect Microbiol. 2021;11:727732. DOI: 10.3389/fcimb.2021.727732.
9. Könönen E, Gursoy M, Gursoy UK. Periodontitis: A Multifaceted Disease of Tooth-Supporting Tissues. J Clin Med. 2019;8(8):1135. DOI: 10.3390/jcm8081135.
10. Köse O, Arabacı T, Yemenoglu H, Ozkanlar S, Kurt N, Gumussoy I, et al. Influence of experimental periodontitis on cardiac oxidative stress in rats: a biochemical and histomorphometric study. J Periodontal Res. 2017;52(3):603–608. DOI: 10.1111/jre.12428.
11. Susana FM, Paula P, Slobodianik N. Dietary modulation of thymic enzymes. Endocr Metab Immune Disord Drug Targets. 2014;14(4):309–12. DOI: 10.2174/1871530314666140915125248.
12. Tomina DC, Petruțiu ȘA, Dinu CM, Crișan B, Cighi VS, Rațiu IA. Comparative Testing of Two Ligature-Induced Periodontitis Models in Rats: A Clinical, Histological and Biochemical Study. Biology (Basel). 2022;11(5):634. DOI: 10.3390/biology11050634.

Publication:

«World of Medicine and Biology» Vol. 21 No. 92 (2025) , с. 165-168
УДК [57.084.1+616-08-039.71]:612.11

DOI:

https://doi.org/10.26724/2079-8334-2025-2-92-165-168