EnglishУкраїнська
  • Main
  • Useful links
  • Information for Contributors
  • About
  • Editorial board

  • Article
    Kotyk T. L.

    MORPHOMETRIC ANALYSIS OF SUBMANDIBULAR GLAND PARENCHYMA OF INTACT RATS USING THE METHODS OF MULTIVARIATE STATISTICS


    About the author: Kotyk T. L.
    Heading EXPERIMENTAL MEDICINE
    Type of article Scentific article
    Annotation Seromucous cells and excretory ducts of intact rats were investigated using morphometry and multivariate statistics methods. Three clusters of terminal portion cells, which characterize the corresponding phases of secretory cycle, were identified by means of cluster analysis. It was determined that seromucous cells, metric parameters of which vary during the secretory cycle, are moving from cluster into the cluster: from cluster 1 into the 2nd – after a certain amount of primary products accumulation; from 2nd cluster into the 3rd – after a sufficient amount of secret synthesis and maturation; from cluster 3 into 1st – after the secretions evacuation. The described above processes pass smooth and parallel. At the same time, the share of #1 cluster cells (those that excrete secret and absorb the required incoming products) equals to the share of #3 cluster cells (those that are ready to secretions evacuation). This fact indicates that the secretory cycle of studied gland terminal portions is to be functionally stable. The principal component analysis allowed to specify the priority of morphological features of intralobular ducts. It was determined that the lumen area of the granular and striated ducts is the most significant, the height of duct epithelial cells less important, and the height of epithelial cells and intercalated ducts lumen area are the least important. The cluster analysis and principal component analysis can serve as a mean of a better salivary gland parenchyma condition assessment (from the side of functional morphology).
    Tags seromucous cells, intralobular ducts, morphometric analysis, cluster analysis, principal component analysis
    Bibliography
    • Gubina-Vakulik G. I. Morfologicheskie osobennosti podnizhnecheljustnoj sljunnoj zhelezy krys pri jeksperimental'noj gipokinezii ih materej / G. I. Gubina-Vakulik, Ju. A. Slin'ko, I. I. Sokolova, N. G. Kolousova // Svіt medicini ta bіologії. – 2013. – №2. – C. 26-28.
    • Eroshenko G.A. Analіz znachushhostі koreljacіjnih zv’jazkіv mіzh morfometrichnimi pokaznikami velikih slinnih zaloz shhurіv pіslja stimuljacії / G. A. Єroshenko // Svіt medicini ta bіologії. – 2012. – №2. – C. 94-97.
    • Korol' T. V. Suchasnі ujavlennja pro sekrecіju ta rol' u nіj kal'cіju / T.V. Korol' // Aktual'nі problemi medicini, farmacії ta bіologії bіologії. – 2006. – №2. – C. 65-72.
    • Mozhejko L. A. O geterogennosti jekzokrinnogo otdela podzheludochnoj zhelezy i ego fiziologicheskih rezervah / L. A. Mozhejko // Zhurnal GrGMU. – 2009. – №4. – C. 66-69.
    • Chajkovs'kij Ju. B. Stovburovі klіtini velikih slinnih zaloz / Ju. B. Chajkovs'kij, S. B. Gerashhenko, O. І. Dєl'cova // Svіt medicini ta bіologії. – 2012. – №2. – C. 200-203.
    • Ali Z. H. Histomorphometric analysis of the postnatal development and growth of rat submandibular glands in offsprings of diabetic mothers / Z. H. Ali, R. Mubarak // Journal of American Science. – 2012. – Vol.8, №1. – P. 342-349.
    • Carpenter G. H. Secretion of IgA by rat parotid and submandibular cells in response to autonomimetic stimulation in vitro / G. H. Carpenter, G. B. Proctor // International Immunopharmacology. – 2004. – Vol.4, №8. – P. 1005-1014.
    • Caldeira E. J. Stereology and ultrastructure of the salivary glands of diabetic Nod mice submitted to long-term insulin treatment. / E. J. Caldeira // The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology. – 2005. – Vol.286, №2. – P. 930-937.
    • Haldar P. Cluster analysis and clinical asthma phenotypes. / P. Haldar, Ian D. Pavord, D. E. Shaw [et al.] // American journal of respiratory and critical care medicine. – 2008. – Vol.178, №3. – P. 218-224.
    • Man Y. G. Contributions of intercalated duct cells to the normal parenchyma of submandibular glands of adult rats. / Y. G. Man, W. D. Ball, L. Marchetti, a R. Hand // The Anatomical record. – 2001. – Vol.263, №2. – P. 202-14.
    • Noorafshan A. Volume-weighted mean volume of the submandibular gland acini in male and female diabetic rats / A. Noorafshan // Micro. – 2006. – Vol.37, №7. – P. 613-616.
    • Peres-Neto P. R. How many principal components? stopping rules for determining the number of non-trivial axes revisited / P. R. Peres-Neto, D. a. Jackson, K. M. Somers // Computational Statistics & Data Analysis. – 2005. – Vol.49, №4. – P. 974-997.
    • Roussa E. Distribution of V-ATPase in rat salivary glands / E. Roussa, F. Thévenod // Eur.J.Morpho. – 1998. – №36. – P. 147-152.
    • Salivary acinar cells from aquaporin 5-deficient mice have decreased membrane water permeability and altered cell volume regulation. / C. M. Krane, J. E. Melvin, H. V. Nguyen [et al.] // The Journal of biological chemistry. – 2001. – Vol.276, №26. – P. 23413-20.
    • Schneider C. A. NIH Image to ImageJ: 25 years of image analysis / C. A. Schneider, W. S. Rasband, K. W. Eliceiri // Nature Methods. – 2012. – Vol.9, №7. – P. 671-675.
    Publication of the article «World of Medicine and Biology» №1(48), 2015 year, 138-142 pages, index UDK 616-002.16+616.316+616-092.9+616-036.8