EnglishУкраїнська
  • Main
  • Useful links
  • Information for Contributors
  • About
  • Editorial board

  • Article
    Shtanyuk E. A., Bezuhlaya A. P., Lyapunov N. A., Mynuhyn V.

    COMPARATIVE STUDY OF THE EFFECTIVENESS OF CERTAIN PREPARATIONS IN THE FORM OF OINTMENTS AND SOLUTIONS FOR STANDARD AND HOSPITAL STRAINS OF BACTERIA


    About the author: Shtanyuk E. A., Bezuhlaya A. P., Lyapunov N. A., Mynuhyn V.
    Heading EXPERIMENTAL MEDICINE
    Type of article Scentific article
    Annotation Introdaction. Treatment of infected wounds is one of the urgent problems of medicine. Leading among pathogens of inflammatory infections are staphylococci and gram-negative bacteria, mainly Pseudomonas aeruginosa and Escherichia coli. There is an increasing incidence of septic complications caused by hospital antibioticresistent strains. Particular danger is epidemic spreading and infection of wounds by Staphylococcus aureus MRSA. For topical treatment of infected wounds in the 1-st phase of wound healing is widely using of antibacterial ointments and solutions on hydrophilic bases, for example, with such antibiotics such as chloramphenicol and ofloxacin, as well as other active ingredients of antibacterial action: dioxydine, nitazol and streptocide (in combination ) and so on. These drugs have been developed and introduced into medical practice in another 70-90 years of the last century. Therefore, an important question is whether they are effective against major modern hospital strains of polyresistent bacteria. The purpose of the study. The election of promising active antibacterial substance for the development of ointments and solutions for local treatment of purulent wounds on a comparative study of antibacterial action of industrial and experimental samples of drugs against the standard strains of S. aureus, E. coli and P. aeruginosa and hospital polyresistent strains of these bacteria. Materials and Methods. We investigated Oflokain-Darnitsya ointment Nitacid-Darnitsya ointment, Dioxizol-Darnitsya solution, Levomekol ointment, ointment 5% Dioxidine and experimental samples of Dioxidine ointment 1.2% and Dioxizol solution with different concentrations dioxidine: 0.25% 0, 50%, 0.75%, 1.00%, 1.25% and 1.50% by agar diffusion method. Antibacterial activity was determined on standard strains of S. aureus ATCC 25923, E. coli ATCC 25922, P. aeruginosa ATCC 27853 and clinical polyresistant strains of S. aureus № 910, E. coli № 6, P. aeruginosa № 16. Results and discussion. All study drugs showed effective or highly effective antibacterial activity against standard strains of bacteria S. aureus ATCC 25923, E. coli ATCC 25922, P. aeruginosa ATCC 27853. All three hospital strains of bacteria in the previous studies wake resistant to ofloxacin, but strain of S. aureus № 910 was highly sensitive, strain of E. coli № 6 was sensitive, strain of P. aeruginosa № 16 was insensitive to Oflokain Darnitsya ointment. All three clinical strains of bacteria were sensitive to the Levomekol ointment and showed high sensitivity to drugs with dioxidine that had an advantage over other investigated ointments. Dioxidine revealed highly antibacterial effect on all 3 clinical studied strains (zone growth delay were more than 25 mm) at concentrations ranging from 1.0% to 1.5%. Сonclusions. Preparations with dioxidine showed highly effective antimicrobial action against antibiotic resistant clinical isolates of bacteria and had the advantage over other studied preparations, particularly with the ointments with ofloxacin and chloramphenicol. It is shown that for the manifestation of highly effective antimicrobial action against hospital strains of bacteria dioxidine should be included in the preparations at concentrations ranging from 1.0% to 1.5% for the topical treatment of wounds.
    Tags antibacterial action, bacteria, dioxidine clinical strains, ointment, solution
    Bibliography
    • Abaev Ju. K. Cpravochnik hirurga. Rany i ranevaja infekcija / Ju. K. Abaev // – Rostov n/D: Feniks, - 2006. – 427 s.
    • Brian L. E. Bakterial'naja rezistentnost' i chuvstvitel'nost' k himiopreparatam: per. s angl. / L.E. Brian // – M.: Medicina, - 1984. – 272 s.
    • Bezuglaja E. P. Teorija i praktika mestnogo lechenija gnojnyh ran / E. P. Bezuglaja, S. G. Belov, V. G. Gun'ko [i dr.] // – K.: Zdorov’ja, - 1995. – 384 s.
    • Bojko V. V. Polirezistentnost' mikroflory v hirurgicheskoj klinike / V. V. Bojko, V. K. Logachov, I. A. Krivoruchko [i dr.] // Harkіvs'ka hіrurgіchna shkola. – 2012. – № 2 (53). – S. 72-73.
    • Blatun L. A. Mozhlivostі suchasnih mazej v lіkuvannі gnіjnih ran, prolezhnіv, trofіchnih virazok / L.A. Blatun // I MEDIC medichnі stattі. – Rezhim dostupa k materialam: http://i-medic.com.ua/index.php?newsid=4444.
    • Voljans'kij Ju. L. Vivchennja specifіchnoї aktivnostі protimіkrobnih lіkars'kih zasobіv: metodichnі rekomendacії / Ju. L. Voljans'kij, І. S. Gricenko, V. P. Shirobokov [ta іn.] // MOZ Ukraїni. – K.: Derzhavnij Farmakologіchnij centr, - 2004. – 38 s.
    • Viznachennja chutlivostі mіkroorganіzmіv do antibakterіal'nih preparatіv: metodichnі vkazіvki, zatverdzhenі MOZ Ukraїni vіd 05.04.07. – K., - 2007. – 9 s.
    • Voljanskij Ju. L. Biologicheskaja harakteristika i mikrobiologicheskaja identifikacija nefermentirujushhih gramotricatel'nyh bakterij: uchebnoe posobie / Ju.L. Voljanskij, V.I. Chernjavskij, S.V. Birjukova [i dr.] // – Har'kov, - 2010. – 47 s.
    • Vіl'canjuk O. A. Harakteristika zbudnikіv gnіjno-zapal'nih procesіv m’jakih tkanin ta pіsljaoperacіjnih gnіjnih uskladnen' u hvorih zagal'no-hіrurgіchnogo stacіonaru / O.A. Vіl'canjuk, M.O. Hutorjans'kij // Harkіvs'ka hіrurgіchna shkola. – 2012. – № 2 (53). – S. 84-88.
    • Glanc S. Mediko-biologicheskaja statistika / S. Glanc // – Moskva: - 1999. – 360 s.
    • Gostishhev V. K. Infekcii v hirurgii / V. K. Gostishhev // – M.: GJeOTAR-Media, - 2007. – 760 s.
    • Gosudarstvennyj reestr lekarstvennyh sredstv Rossii. Rezhim dostupa k materialam: http://grls.rosminzdrav.ru.
    • Dacenko B. M. Metodicheskie rekomendacii po jeksperimental'nomu (doklinicheskomu) izucheniju lekarstvennyh preparatov dlja mestnogo lechenija ran / B. M. Dacenko, S. V. Birjukova, T. I. Tamm [i dr.] // MZ SSSR, M.-1989.-47 s.
    • Dmitrenko O. A. Osobennosti jepidemicheskogo rasprostranenija meticillin-rezistentnyh Staphylococcus aureus v stacionarah RF. Analiz tendencij i vozmozhnye puti reshenija problemy / O. A. Dmitrenko, I. A. Shilov, V. G. Lunin [i dr.] // Materialy II Mezhdunarodnogo kongressa po vnutribol'nichnym infekcijam, Moskva, 23-24 nojabrja - 2011 g.
    • Kuzin M. I. Rany i ranevaja infekcija: rukovodstvo dlja vrachej / M. I. Kuzin, B. M. Kostjuchenok // – 2-e izd., pererab. i dop. – M.: Medicina, - 1990. – 592 s.
    • Kovalenko V. N. KOMPENDIUM 2011 – lekarstvennye preparaty / V. N. Kovalenko, A. P. Viktorova // – K.: MORION, - 2011. – 2320 s.
    • Lupal'cov V. I. Aktual'nye voprosy v reshenii problemy vnutrigospital'noj infekcii / V. I. Lupal'cov // Ukraїns'kij zhurn. hіrurgії. – 2008. – № 1. – S. 26-28.
    • Metodicheskie ukazanija po mikrobiologicheskoj diagnostike zabolevanij, vyzyvaemyh jenterobakterijami. Prikaz MZ SSSR ot 17.04.1984 № 04 – 723/3-84. – 23 s.
    • Padejskaja E. N. Antibakterial'nyj preparat dioksidin: itogi i perspektivy primenenija v klinicheskoj praktike / E. N. Padejskaja // Novye lekarstvennye preparaty: Jekspress-inform. – M.: VNIISJeNTI, -1989. – № 7. – S. 1-18.
    • Siplivij V. O. Polіrezistentnіst' sered zbudnikіv hіrurgіchnih іnfekcіj / V. O. Siplivij, A. Ja. Ciganenko, K. V. Kon' [ta іn.] // Harkіvs'ka hіrurgіchna shkola. – 2012. – № 2 (53). – S. 80-83.
    Publication of the article «World of Medicine and Biology» №2(50) 2 part 2015 year, 189-194 pages, index UDK 579.8:615.28:615.33:615.451:615.454.1:57.085.2