| About the author: |
Gerush І. V., Cherniukh О. G., Dikal М. V. |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
A subject of study in this work is exploration of main biochemical indices of the blood plasma of albino rats with the background of induced diabetes mellitus (DM). Alloxan DM was modeled in rats by intraperitoneal injection of a 5% liquid of alloxan monohydrate in the dose of 150 mg/kg of body weight. On the 14th day of the experiment, the changes in the indices were compared with the control data . The results of our studies have found a pronounced hyperglycemia ranging within 11-20 mmol/L. An average glucose concentration was 12,6 ± 1,28 mmol/L that was twice as much as compared to the similar index in the group of control animals 6,0 ± 0,13 mmol/L. A significant increase in AST and GGT activity was observed, respectively, by 84% (p <0.01) and 79% (p<0.01), while the increase in ALT activity was slightly less – by 36% (p<0.05). It has been established that against a background of significant hyperglycemia (12.6 ± 1.28 mmol/L), the change in enzyme activity is a more sensitive indicator of the acute phase of alloxan DM development than such generally accepted indicators as the concentration of total protein, albumin, urea, creatinine. Furthermore, the enzyme activity ratios may indicate necrotic disorders of the functional state of the liver, as the primary organ responsible for rearranging the metabolic pathways due to the lysis of the insulin-producing cells. |
| Tags |
alloxan's diabetes mellitus, blood plasma, biochemical indices, albino rats |
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| Publication of the article |
«World of Medicine and Biology» №4(62), 2017 year, 123-126 pages, index UDK 616.153: 616.379-008.64]-092.9 |
| DOI |
10.26724/2079-8334-2017-4-62-123-126 |
