Cheremisina V. F., Bereznyakova A. I.

CORRELATION OF CYTOKINE PRODUCTS LEVELS IN REGULATION MECHANISMS OF BONE TISSUE REMODELING PROCESSES IN PARODONTITIS

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About the author:	Cheremisina V. F., Bereznyakova A. I.
Heading	EXPERIMENTAL MEDICINE
Type of article	Scentific article
Annotation	The levels of production of cytokines and their interrelations in the mechanisms of regulation of bone tissue remodeling processes on the model of periodontitis accompanied by inflammation were studied. The study was carried out on two groups of non-linear white male rats. In the blood serum the levels of RANKL, osteoprotegerin, TGFβ1, IL-1 Ra, adiponectin, visfatin and IL-17 were determined by an enzyme immunoassay. In animals with periodontitis, the levels of RANKL and TGFβ1 decreased and the levels of IL-1 Ra and IL-17 increased. Differences in the nature of the relationship between cytokine levels in the study groups were found. The characteristics of the cytokine profile revealed reflect the end of the active resorption phase initiated by periodontitis and inflammation, and the beginning of the phase of compensatory reactions in bone tissue. The disappearance of interrelations in the system of normal regulation of bone tissue remodeling between pairs of cytokines and their appearance between other pairs testifies to violations in the work of regulatory mechanisms in periodontitis accompanied by inflammation.
Tags	bone tissue, remodeling, cytokines, regulation
Bibliography	Lapach SN, Chubenko PN, Babich AV. Statisticheskiye metody v mediko–biologicheskikh issledovaniyakh s ispolzovaniyem Exel. K. : MORION, 2000. – 320 s. [in Russian] Obshchiye eticheskiye printsipy eksperimentov na zhivotnykh : materialy I Natsionalnogo kongressa po bioetike, 2001. – K.: NANU. – 16 s. [in Russian] Peshkova LV. Spontannoye porazheniye tkaney parodonta u krys v usloviyakh vivariya, kak model parodontita. Vísnik stomatolohiyi: 1997; 2: 163–168. [in Russian] Biver E, Sallio C, Combescure C. Influence of adipokines and ghrelin on bone mineral density and fracture risk: a systematic review and meta-analysis. J. Clin. Endocrinol. Metab. 2011; 96(9): 2703–2713. Cheung WW, Zhan IW, Paik KH. The impact of inflammation on bone mass in children. Pediatr. Nephrol. 2011; 26 (11): 1937–1946. Kanozawa L, Vamaguchi T, Vano S. Adiponectin and AMP kinase activator stimulate proliferation, differentiation and mineralization of osteoblastic MC3T3-E1 cells. BMC Cell. Biol. 2007; 8: 51. Lua XH, Guo LI., Xie H. Adiponectin stimulates RANKL and inhibits OPG expression in human osteoblasts through the MAPK signaling pathway. J. Bone Miner. Res. 2006; 21 (10): 1648–1656. Tu Q, Zhang J, Dong LQ. Adiponectin inhibits osteoclastogenesis and bone resorption via APPL1-mediated suppression of Akt 1. J. Biol. Chem. 2011; 286 (14): 12542–12553. Vamaguchi N, Kukita T, Li V. Adiponectin inhibits induction of TNF-alpha/RANKL-stimulated NFATc1 via the AMPK signaling. FEBS Lett. 2008; 582 (3): 451–456. Zwerina I, Redlich K, Polzer K. TNF-induced structural joint damage is mediated by IL-1. Proc. Natl. Acad. Sci. USA. 2007; 104 (28): 11742–11747.
Publication of the article	«World of Medicine and Biology» №2(64), 2018 year, 181-185 pages, index UDK 616.71-007.234-076:616.61-036
DOI	10.26724/2079-8334-2018-2-64-181-185