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    S.O. Zubchenko, V.V. Chopyak, A.M. Havrylyuk, G.O. Potemkina, M.P. Lomikovska

    INVESTIGATION OF MIR-BART 13 AND 15 IN PATIENTS WITH ALLERGOPATHY IN COMBINATION WITH CHRONIC EPSTEIN-BARR VIRAL INFECTION


    About the author: S.O. Zubchenko, V.V. Chopyak, A.M. Havrylyuk, G.O. Potemkina, M.P. Lomikovska
    Heading CLINICAL MEDICINE
    Type of article Scentific article
    Annotation Epstein-Barr (EBV) virus initiates a wide spectrum of immunomodulating effects in the human body. EBV is one of the first viruses, in which expression of miRNAs has been revealed, that are used as targets for detection of the virus in clinical investigations. To investigate association relationships of miR-BART 13 and 15 in patients with allergopathy and chronic EBV-infection in active and latent phases. In general, 46 patients with chronic EBV-infection in active/latent phases have been examined, age 18-59 years, 65% of females, 37% of males. Clinical, general laboratory, instrumental, specific allergological, molecular genetic, and cytological investigations were performed. High levels of miR-BART 13 expression were detected in patients with allergopathy in combination with chronic EBV-infection in active (р<0.01) and latent (р<0.05) phases compared to healthy individuals. The difference in miRNAs 13, 15 expression levels depending on the medium of virus replication was not found. Investigation of miRNAs 13, 15 in the groups, formed according to verified allergic diseases, showed that higher levels of miRNAs 13 were observed in the group of patients with bronchial asthma (BA) in combination with an active phase of EBV-infection (p<0.01). Reverse correlation between miR-BART-13 and FEV1 and direct correlation with levels of total IgE were also detected in this group of patients. Higher indices of miR-BART 15 were recorded in patients with BA and active EBV-infection compared to control group (p<0.05). In patients with BA and active phase of chronic EBV-infection, elevation of the levels of miR-BART-13 expression can be a biomarker of the severity of allergic inflammatory process due to amplification of bronchial obstruction level and formation of hyper-IgE syndrome.
    Tags Chronic Epstein-Barr viral infection, allergic diseases, miR-BART 13, miR-BART 15
    Bibliography
    • Gourzones C, Gelin A, Bombik I, Klibi J, Verillaud B, Gnigay J. Extra-cellular release and blood diffusion of BART viral micro-RNAs produced by EBV-infected nasopharyngeal carcinoma cell. Virol. J. 2010;7:270-272.
    • Grotto I, Mimouni D, Huerta M, Mimouni M, Cohen D, Robin G, Pitlik S, Green S. Clinical and laboratory presentation of EBV positive infectious mononucleosis in young adults. Epidemiol Infect. 2013;131 (1):683-689.
    • Kawano Y, Iwata S, Kawada J, Gotoh K, Suzuki M, Torii Y, Kojima S, Kimura H, Ito Y. Plasma viral microRNA profiles reveal potential biomarkers for chronic active Epstein-Barr virus infection. J Infect Dis. 2013;208(5):771-9.
    • Komabayashi Y, Kishibe K, Nagato T, Ueda S, Takahara M, Harabuchi Y. Circulating Epstein-Barr virus-enencoded micro-RNAs as potential biomarkers for nasal natural killer/T-cell lymphoma. Hematol Oncol. 2017;35(4):655-663.
    • Komabayashi Y, Kishibe K, Nagato T, Ueda S, Takahara M, Harabuchi Y. Downregulation of miR-15a due to LMP1 promotes cell proliferation and predicts poor prognosis in nasal NK/T-cell lymphoma. Am J Hematol. 2014;89(1):25-33.
    • Lo AK, Dawson CW, Jin DY, Lo KW. The pathological roles of BART miRNAs in nasopharyngeal carcinoma. J. Pathol. 2012;227:392-403.
    • Pizzigallo E, Racciatti D, Gorgoretti V. EBV Chronic Infections. Mediterr J Hematol Infect Dis. 2010;2(1):e2010022.
    • Rojas JM, Avia M, Martin V, Noemi S. IL-10: a multifunctional cytokine in viral infections. J Immunol Res. 2017;17:6104054.
    • Sarah R, Gonzales-van Horn, David Farrar. Interferon at the crossroads of allergy and viral infections. J Leukoc Biol. 2015;98(2):185-194.
    • Skinner CM, IvanovNS, Barr S.A, Chen Y, Skalsky RL. An Epstein-Barr virus microRNA blocks interleukin-1 (IL-1) signaling by targeting IL-1 receptor 1. J Virol. 2017;91(21):74-78.
    • Sung IY, Brandi CJ, Naomi JL, Bethany DH, Srilalitha K. Epstein-Barr Virus IL-10 engages IL-10R1 by a two-step mechanism leading to altered signaling properties. J Biol Chem. 2012; 287(32):26586-26595.
    • Wang RC, Chang ST, Hsieh YC, Huang WT, Hsu JD. Spectrum of Epstein-Barr virus-associated T-cell lymphoproliferative disorder in adolescents and young adults in Taiwan. Int J Clin Exp Pathol. 2014;7(5):2430-7.
    • Yang P, Zhiyang N, Yang Z, Kuo Z, Jinming L. Identification of EBV infection in adults with egg specific food allergy. Virol J. 2013;10:9.
    • Yu-Jin Jung, Hoyun C, Hyoji K, Suk KL. MicroRNA miR-BART20-5p stabilizes Epstein-Barr Virus latency by directly targeting BZLF1 and BRLF1. J Virol. 2014;88(16):9027-9037.
    • Zubchenko SO, Maruniak SR. Influence of herpes virus infections on the formation of allergopathy. East European Scientific Journal. 2016;1(10):15-20.
    Publication of the article «World of Medicine and Biology» №2(68), 2019 year, 054-060 pages, index UDK 616-056.3-02:[616.98:578.825.13]-07
    DOI 10.26724/2079-8334-2019-2-68-54-60