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    N.S. Khopta, L.Ya. Shpilchak

    POSITIVE INFLUENCE OF ARTICHOKE EXTRACT ON STRUCTURAL AND METABOLIC PROCESSES IN BONE TISSUE OF RATS CONDITIONED UPON CADMIUM-NITRIC INTOXICATION


    About the author: N.S. Khopta, L.Ya. Shpilchak
    Heading EXPERIMENTAL MEDICINE
    Type of article Scentific article
    Annotation The purpose of the study was to investigate the changes in the histological structure, bioelement compositionand markers of bone metabolism in the blood of white male rats with experimental cadmium-nitrite intoxication and the use of artichoke extract for correction of disturbances that arose under the influence of toxicants. It has been established that on the background of combined action of CdCl2and NaNO2in animal femoral bones, osteoclastic resorption processes over osteosynthesis predominate and ash content of the most important osteotropic elements (Ca, Zn and Cu significantly decrease), while cadmium content increases greatly. Indicators of phosphorous-calcium metabolism change in blood plasma, the activity of acid phosphatase increases and oxyproline concentration increases, alkaline phosphatase activity decreases. Artichoke extract positively influences on reparative processes in bones. With the administration of artichoke extract the balance of macro- and micronutrients is restored in the femur of the animals, cadmium is significantly reduced. Biochemical markers of bone metabolism are normal in the blood plasma, the values of which do not significantly differ from those of intact ones (with the exception of the concentration of oxyproline that remains higher) to the end of the experiment.
    Tags artichoke extract, cadmium-nitrite intoxication, histological structure of femoral bones, markers of bone metabolism
    Bibliography
    • Antonyak HL, Babych NO, Biletska LP, ta in. Kadmiy v orhanizmi lyudyny i tvaryn. Vplyv na funktsionalnu aktyvnist orhaniv i system. Studia Biologica. 2010; 4 (3): 125-36. [in Ukrainian]
    • Beier EE, Maher JR, Sheu TJ, et al. Heavymetal lead exposure, osteoporotic-like phenotype in an animalmodel, and depression of Wnt signaling. Environ. Health Perspect. 2013; 121(1): 97-104.
    • Cronin M. Metals, Toxicity and Oxidative Stress. Current Medicinal Chemistry. 2015;12(10). 1161-208. doi: http://doi.org/10.2174/0929867053764635
    • European convention for the protection of vertebrate animals used for experimental an others scientific purposes/ Strasbourg: Council of Europ, 1986. No 123.52p.:http://zakon.rada.gov.ua/cgi-bin/laws/main.cgi?nreg=994_137.
    • Heidarian E,Rafieian-Kopaei M. Protective effect of artichoke (Cynara scolymus) leaf extract against lead toxicity in rat.Pharm Biol.2013. Sep;51(9):1104-9.
    • Lelovas PP, Xanthos TT, Thoma SE,et al.The laboratoryrat as an animalmodel for osteoporosis research.Comparativa medicine. 2008,58(4):  424-30.
    • Moulis J-M. Cellular mechanisms of cadmium toxicity related to the homeostasis of essential metals. BioMetals. 2010;23(5):877-96. doi: http://doi.org/10.1007/s10534-010-9336-y.
    • Nilforoushan D, Gramoun A, Glogauer M, et al. Nitric oxide enhances osteoclastogenesis possibly by mediating cell fusion. Nitric Oxide. 2009; 21(1): 27-36.
    • Ostrovska SS. The influence of heavy metals on the content of Calcium and Alkaline Phosphatase in the Rats’ blood. Visnyk problem biolohiyi i medytsyny 2014; 4(114): 169-71.
    • Thévenod F, Lee W-K. Toxicology of Cadmium and Its Damage to Mammalian Organs // Metal Ions in Life Sciences. 2013; 11: 415-90. doi: http://doi.org/10.1007/978-94-007-5179-8_14 .
    • Vasko LV, Kiptenko LI, Hortitskaya OM. Ultrastructure of bone cells under conditions of irradiation and use of salts of heavy metals. World of Medicine and Biology. 2011. 4: 23-6.
    Publication of the article «World of Medicine and Biology» №2(68), 2019 year, 204-209 pages, index UDK 615.322 + 591.84+ 577.12 +546.48 + 546.173
    DOI 10.26724/2079-8334-2019-2-68-204-209