| About the author: |
T. Yu. Kvitnitskaya-Ryzhova, S. P. Lugovskyi, P. P. Klymenko, S. A. Mykhalskiy, S. P. Malysheva, O. S. Tkachenko, H. V. Kosiakova |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
Histological, histochemical and morphometric changes of the pancreas in young (4 months) and old (16 months) rats in the modeling of insulin resistance and its correction with N-Stearoylethanolamine were studied. In the experiment, insulin resistance was reproduced by a long-term, 6 months, keeping rats on a fat diet (fat content in the diet – 58 %), and its development was monitored by glycemic control. To correct insulin resistance, an aqueous suspension of NSE was used, which was administered to rats per os at a dose of 50 mg/kg for 2 weeks. Two-factors analysis of variance proved the leading role of age in changing the basic morphometric parameters that characterize the development of destructive changes in the pancreatic islets (number of apoptotic cells), inflammatory changes in pancreatic tissue (number of mononuclear cells in the stroma), and secretory dysfunction of ß-insulocytes (specific number of insulocytes with aldehyde-fuchsin -positive secretory granules). The contribution of the age factor to changes in these indices is 78.3 %, 56.1 % and 41.5 %, respectively. It was found that the number of apoptotic TUNEL-positive cells in the pancreatic islets is an informative integral index for the assessment of destructive changes in insulocytes, which should be recommended when screening for pharmacological correction of insulin resistance and diabetes. |
| Tags |
pancreatic islets, rats, insulin resistance, apoptosis, pharmacological correction |
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| Publication of the article |
«World of Medicine and Biology» №2(80), 2022 year, 204-209 pages, index UDK 616.379-008.64:611.37:612.67 |
| DOI |
10.26724/2079-8334-2022-2-80-204-209 |
